Active-Italia: Total polyphenolic fraction (TPF) – Assessment of the antioxidant activity of an olive oil total polyphenolic fraction and hydroxytyrosol from a Greek Olea europea variety in endothelial cells and myoblasts

Total polyphenolic fraction (TPF) – Assessment of the antioxidant activity of an olive oil total polyphenolic fraction and hydroxytyrosol from a Greek Olea europea variety in endothelial cells and myoblasts

Cat: , , , , , , , , , ,
Olive oil constitutes the basis of the Mediterranean diet, and it seems that its biophenols, such as hydroxytyrosol may scavenge free radicals, attracting distinct attention due to their beneficial effects in many pathological conditions, such as cancer. To the best of our knowedge, this is the first study in which the functional properties of an Olive Oil total polyphenolic fraction (TPF) and pure hydroxytyrosol were examined in order to determine their antioxidant effects at a cellular level in endothelial cells and myoblasts.

The test compounds were isolated using a green gradient-elution centrifugal partition chromatography-based method that allows the isolation of large volumes of OO in a continuous extraction procedure and with extremely low solvent consumption. For the isolation of HT, a combination of two chromatographic techniques was used, which is effective for the recovery of pure compounds from complex natural extracts. Moreover, TPF and HT exhibited potent free radical scavenging activity in vitro. The cells were treated with non-cytotoxic concentrations and their redox status [in terms of glutathione (GSH) and reactive oxygen species (ROS) levels] was assessed. TPF extract was less cytotoxic than HT, and the observed differences between the two cell lines used suggest a tissue-specific activity. Finally, flow cytometric analysis revealed that both TPF and HT improved the redox status by increasing the levels of GSH, one of the most important antioxidant molecules, in both endothelial cells and myoblasts, while the ROS levels were not significantly affected.


Aerobic organisms are exposed to reactive oxygen species (ROS) due to aerobic metabolism. ROS, such as superoxide anion (O2•−), hydrogen peroxide (H2O2) and hydroxyl radicals (OH), have coalescent chemical properties that confer reactivity to a number of biological targets …

Source of the Article

By Kouka P, Priftis A, Stagos D, Angelis A, Stathopoulos P, Xinos N, Skaltsounis AL, Mamoulakis C, Tsatsakis AM, Spandidos DA, Kouretas D


Click on the icon to read more

Linked Article

Scientific Opinion on the substantiation of health claims related to polyphenols . . .
Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on. . .
find out more
The total polyphenolic fraction (TPF) extracted from virgin olive oil of Italian . . .
Virgin olive oil contains polyphenols, whose amount varies depending on olive cultivar, environmental factors and olive milling process.
find out more
Hydroxytyrosyl: Antioxidant and Skin Regenerative Properties
Since olive oil is used in the preparation of many cosmetic formulations, we explored the antioxidant capacity of HtyOle in human keratinocytes.. . .
find out more
Role of the Mediterranean Diet in the brain and neurodegenerative diseases
Comprehensively provides an overview of the effects of the Mediterranean diet on the brain and its beneficial effects in neurodegenerative diseases.
find out more
Hydroxytyrosol as a Component of the Mediterranean Diet and Its Role in Disease . . .
Hydroxytyrosol is a polyphenol that forms part of the minor compound fraction of extra virgin olive oil, one of the most important elements in the. . .
find out more
The Extra-Virgin Olive Oil Polyphenols Oleocanthal and Oleacein counteract . . .
Inflammation of the adipose tissue plays an important role in the development of several chronic diseases associated with obesity. Polyphenols of. . .
find out more
Oleocanthal as a Dual c-MET-COX2 Inhibitor for the Control of Lung Cancer
Lung cancer (LC) represents the topmost mortality-causing cancer in the U.S. LC patients have overall poor survival rate with limited available. . .
find out more
The phenolic compounds of olive oil: structure, biological activity and . . .
The Mediterranean diet is rich in vegetables, cereals, fruit, fish, milk, wine and olive oil and has salutary biological functions. Epidemiological. . .
find out more
Olive Oil Lipophenols Induce Insulin Secretion in 832/13 β-Cell Models
Glycemic control is a mainstay of type 2 diabetes mellitus (T2DM) clinical management. Despite the continuous improvement in knowledge and progress in. . .
find out more
Olive Oil Phenols
Olive oils contain numerous substances that have a beneficial role in human health. Phenols are natural compounds that are present in extra-virgin. . .
find out more
Oleocanthal from Olea europaea
The Virgin Olive Oil phenolic compounds include about 30 molecules from different chemical classes: phenolic alcohols, such as hydroxytyrosol and. . .
find out more
Oleacein from Italian cultivar ‘Coratina’
Oleacein, and oleocanthal has attracted great attention in recent times because of the interesting biological properties of these molecules. The. . .
find out more



  • The info presented in this post should not replace professional medical or dietary advice, diagnosis or treatment.
  • Always consult your registered dietitian or physician before making any significant dietary or exercise changes.
  • Don’t ignore professional medical advice due to the info presented here.
  • Active-Italia products are intended for laboratory use only and are not for consumer use.

Get Insights on Developments in Biomedical Research

There’s an ocean of publications in biomedical research. We want to help you navigate through them, by bringing you relevant information suited to your professional needs and interests.