Search
Close this search box.

Active-Italia: Extra virgin olive oil rich in polyphenols modulates VEGF-induced angiogenic responses by preventing NADPH oxidase activity and expression

Extra virgin olive oil rich in polyphenols modulates VEGF-induced angiogenic responses by preventing NADPH oxidase activity and expression

Cat: , , , , , , , , , ,
Previous studies have shown the antiinflammatory, antioxidant and antiangiogenic properties by pure olive oil polyphenols; however, the effects of olive oil phenolic fraction on the inflammatory angiogenesis are unknown. In this study, we investigated the effects of the phenolic fraction (olive oil polyphenolic extract, OOPE) from extra virgin olive oil and related circulating metabolites on the VEGF-induced angiogenic responses and NADPH oxidase activity and expression in human cultured endothelial cells.

We found that OOPE (1–10 μg/ml), at concentrations achievable nutritionally, significantly reduced, in a concentration-dependent manner, the VEGF-induced cell migration, invasiveness and tube-like structure formation through the inhibition of MMP-2 and MMP-9. OOPE significantly (P<0.05) reduced VEGF-induced intracellular reactive oxygen species by modulating NADPH oxidase activity, p47phox membrane translocation and the expression of Nox2 and Nox4. Moreover, the treatment of endothelial cells with serum obtained 4 h after acute intake of extra virgin olive oil, with high polyphenol content, decreased VEGF-induced NADPH oxidase activity and Nox4 expression, as well as, MMP-9 expression, as compared with fasting control serum. Overall, native polyphenols and serum metabolites of extra virgin olive oil rich in polyphenols are able to lower the VEGF-induced angiogenic responses by preventing endothelial NADPH oxidase activity and decreasing the expression of selective NADPH oxidase subunits. Our results provide an alternative mechanism by which the consumption of olive oil rich in polyphenols may account for a reduction of oxidative stress inflammatory-related sequelae associated with chronic degenerative diseases.

Source of the Article

Nadia Calabriso, Marika Massaro, Egeria Scoditti, Simona D’Amore, Antonio Gnoni, Mariangela Pellegrino, Carlo Storelli, Raffaele De Caterina, Giuseppe Palasciano, Maria Annunziata Carluccio. ISSN 0955-2863 .

Article

Click on the icon to read more

Linked Article

The total polyphenolic fraction (TPF) extracted from virgin olive oil of Italian . . .
Virgin olive oil contains polyphenols, whose amount varies depending on olive cultivar, environmental factors and olive milling process.
find out more
Role of the Mediterranean Diet in the brain and neurodegenerative diseases
Comprehensively provides an overview of the effects of the Mediterranean diet on the brain and its beneficial effects in neurodegenerative diseases.
find out more
Mediterranean Diet in Preventing Neurodegenerative Diseases
Most, but not all, epidemiologic studies report a protective association between MD adherence, cognitive impairment, and brain health. Data from. . .
find out more
Health-promoting properties of oleocanthal and oleacein: Two secoiridoids from . . .
Extra virgin olive oil (EVOO) polyphenols, including the secoiridoids oleocanthal (OLC) and oleacein (OLE), are attracting attention because of their. . .
find out more

Share

IMPORTANT DISCLAIMERS

  • The info presented in this post should not replace professional medical or dietary advice, diagnosis or treatment.
  • Always consult your registered dietitian or physician before making any significant dietary or exercise changes.
  • Don’t ignore professional medical advice due to the info presented here.
  • Active-Italia products are intended for laboratory use only and are not for consumer use.

Get Insights on Developments in Biomedical Research

There’s an ocean of publications in biomedical research. We want to help you navigate through them, by bringing you relevant information suited to your professional needs and interests.